96P Goblet cell differentiation in colorectal cancer

In the large intestine, multipotent stem cells are located at base of crypt and differentiate into three main cell types: enterocytes, goblet cells, enteroendocrine cells. Goblet cells’ function is synthesis secretion mucins. Genetic epigenetic changes that provide survival advantages for or progenitor resulting in deregulation cellular differentiation major causes all carcinomas. Our laboratory has a collection colorectal cancer (CRC) lines, well characterised terms gene expression mutations. We analysed presence CRC lines using genes Mucin 2 (MUC2) Trefoil factor 3 (TFF3). The both mRNA level protein were investigated. effects various transcription factors assessed by knockdown overexpression techniques. found most unable to produce number MUC2 TFF3-positive among positive was quite variable. While normal colon, TFF3 always co-expressed, but not case lines. MUC2-negative appear reflect novel interesting subset. investigation several on showed downregulation Atonal homologue 1 (ATOH1) had dramatic effect production, while knocking down SAM pointed domain ETS (SPDEF), Caudal type homeobox (CDX1), (CDX2) modest effect. Individually, none these sufficient trigger differentiation. As conclusion, percentage differs substantially between Classification reveals an subset without expressing MUC2. ATOH1, SPDEF, CDX1, CDX2 significant differentiation, their own, they induce Understanding mechanisms important advances prevention treatment CRC.